 | Nathaniel Ayers (1788-1841) 
|
 | Milcah Elizabeth Walters (1795-1854)
|
| Elizabeth M. Ayers (1817-1843)  |
| Lucy Ayers (1818-1840) |
| Moses Payne Ayers (1819-1862) |
 | Judith Pamela Ayers (1827-1903) |
| Eliza Abigail Ayers (1829-1882) |
| Charles Franklin Ayers (1830-1889) |
| Robert Wiley Ayers (1832-1917) |
 | Jeremiah Walters Ayers (1824-1885)
|
| Louisa Emmaline Langston (1835-1912) |
 | Emma Elizabeth Ayers (1879-1963) |
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